Mica Microhub

Everyone can now leverage microscopy to make more discoveries.

Mica provides a clear sample overview and allows you to easily change observation conditions with just a few clicks.

• 85% fewer steps to the first image
• 33% less time to the first image
• 50% of the training time

Mouse embryo (E15.5) cryosection captured with the PL APO 20x/0.75 CS2 objective. Section shows Tbr2 cells labeled with CF488A, Satb2 cells labeled with CF555 and Ctip2 cells with CF633 plus nuclei counterstaining with DAPI. The acquisition of two sections took less than 5 minutes, while previously it took 2 hours on the lab’s comparison device. Sample and images are courtesy of Giulia Di Muzio at the lab of Dr. Pei-Chi Wei at the DKFZ, Heidelberg, Germany.

The Microhub enables you to simultaneously capture all four labels of different structures in a single acquisition for widefield or confocal, without ever moving your sample. This overcomes the spatiotemporal mismatch between labels of moving objects during sequential acquisition. All are powered by our patented FluoSync technology, a fast and gentle method for multicolor fluorescence imaging.

Mica unifies transmitted and fluorescence light imaging modalities. You can select from multiple imaging modalities all within one Microhub, including widefield, confocal, THUNDER imaging, LIGHTNING, Z-stacks, time-lapse and more.

This enables you to

• generate fast overviews with widefield at low magnification
• gradually zoom in on the regions of interest
• switch to confocal when and where needed without ever moving the sample to a different system

Live cell experiments require the cells to be in optimal shape. Typically, 2D and 3D cells in media require the temperature and the pH (via CO2) in the environment to be controlled. Stable nutrition and ion concentrations require the evaporation to be minimal. Some experiments even demand the O2 to be mimicked closer to physiological levels. Mica can provide the right conditions in the live cell configuration.

• Mica is an incubator: The entire encapsulated inner sample space can be climate controlled (temperature, CO2 and humidity regulation) and offers ideal conditions for short and long-term live cell observation.
• From dark to light: Mica also enables you to enjoy a brightly lit lab—freeing you from the constraints of sitting in a dark room for hours monitoring your experiment.

Formation of 3D spheroids from 1000 stably transfected MDCK MX1-GFP cells per well (left half) and 1000 U2OS cells per well (right half). Time-lapse acquisition over 60 hours, with 30-minute intervals. Green, GFP. Gray, integrated modulation contrast.

Intelligent automation and AI-supported analysis enable greater efficiency and a faster track to publication.

• Reduce over 60% of process steps through system intelligence
• Reduce time and effort from sample to insight by simplifying your entire workflow
• Enable 100% reproducibility and repeatability throughout your experiment

U2OS cells were labelled with SiR-Actin, TMRE (mitochondria activity), CellEvent™ (caspase activity), and DAPI (nuclei). 63x magnification, widefield mode.