Gene Modification and Analysis in CRISPR-Mediated Gene Therapy
The delivery of gRNA and Cas reagents can vary depending on the recipient cell line. Automate your chemical transfection process and ensure consistency with aBeckman Coulter Life Sciences Biomek i-Series Integrated Workstation.
Post-modification, assess cell counts and viability using a Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer. More in-depth analyses, such as mitotic analyses and fluorescent imaging, can be performed on a Leica Microsystems STELLARIS Confocal Microscope Platform or automated on a Leica Microsystems Mica Microhub. Automatically capture and analyze these data, including imaging and other fluorescent assays, using the Genedata Biopharma Platform. With the software, register every component of a CRISPR-mediated gene therapy, including Cas and sgRNA-encoding plasmids, delivery systems (e.g., LNPs, viral vectors) and purified final products.
Collect and track experiment and instrument data with the IDBS Polar Biopharma Lifecycle Management software.